Article
Cloning, expression, and nucleotide sequence of a mutant glgC gene from Escherichia coli B.
Journal of bacteriology - 1 Jul 1992
Meyer C R, Ghosh P, Remy E, Preiss J
Abstract excerpt
A mutant glgC gene contained in a 10.9-kb PstI fragment was cloned from the Escherichia coli B strain SG5 via colony hybridization by using a wild-type glgC probe. The altered allosteric properties of the expressed ADPglucose synthetase were found to result from the conversion of proline to serine at amino acid residue 295.
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Escherichia coli
- Gene Expression
- Genes, Bacterial
- Glucose-1-Phosphate Adenylyltransferase
- Kinetics
- Molecular Sequence Data
- Mutation
- Nucleotidyltransferases
- Plasmids