Article
Isolation of a gramicidin S hyperproducing strain of Bacillus brevis by use of a fluorescence activated cell sorting system.
Applied microbiology and biotechnology - 1 Nov 1992
Azuma T, Harrison G I, Demain A L
Abstract excerpt
A gramicidin S (GS) hyperproducing mutant of Bacillus brevis was isolated by using a protein-staining fluorescence dye (fluorescein isothiocyanate, FITC), and a fluorescence-activated cell sorting system (FACS). By flow cytometry (FCM) analysis after staining with FITC, higher producing cells of the wild-type had higher fluorescence signals than cells with low productivity or cells from a GS non-producing mutant....
Topics
- Bacillus
- Flow Cytometry
- Fluorescein-5-isothiocyanate
- Gramicidin
- Mutation
- Staining and Labeling