Article
Dynamics of IS-related genetic rearrangements in resting Escherichia coli K-12.
Molecular biology and evolution - 1 Mar 1995
Naas T, Blot M, Fitch W M, Arber W
Abstract excerpt
An analysis of restriction fragment length polymorphism (RFLP) using eight residential insertion sequence (IS) elements as hybridization probes reveals that the genome of resting bacteria is more dynamic than it was long believed. Escherichia coli strains stored in agar stabs for up to 30 yr accumulate a genetic variation which is correlated to time of storage. This spontaneous mutagenesis is often IS-specific,...
Topics
- DNA Probes
- DNA Transposable Elements
- Escherichia coli
- Gene Rearrangement
- Genetic Variation
- Mutagenesis
- Nucleic Acid Hybridization
- Pedigree
- Polymorphism, Restriction Fragment Length
- Time Factors