Article
Composite PAGE: an alternate method for increased separation of amplified short tandem repeat alleles.
International journal of legal medicine - 1 Jan 1993
Lorente M, Lorente J A, Wilson M R, Budowle B, Villanueva E
Abstract excerpt
Genetic typing of short tandem repeat (STR) loci may require electrophoretic separation techniques which, depending on the locus, can resolve alleles differing in size by only 2, 3 or 4 base pairs (bps). Many such loci can be separated by horizontal, discontinuous polyacrylamide gel electrophoresis (hd-PAGE). However, some loci present particular separation challenges. A composite (or step) gel system consisting...
Topics
- Actins
- Alleles
- DNA
- Electrophoresis, Polyacrylamide Gel
- Equipment Design
- Gene Amplification
- Humans
- Myelin Basic Protein
- Repetitive Sequences, Nucleic Acid
