Article
Four rice seed cDNA clones belonging to the alpha-amylase/trypsin inhibitor gene family encode potential rice allergens.
Bioscience, biotechnology, and biochemistry - 1 Jul 1995
Alvarez A M, Fukuhara E, Nakase M, Adachi T, Aoki N, Nakamura R, Matsuda T
Abstract excerpt
Four rice seed proteins encoded by cDNAs belonging to the alpha-amylase/trypsin inhibitor gene family were overexpressed as TrpE-fusion proteins in E. coli. The expressed rice proteins were detected by SDS-PAGE as major proteins in bacterial cell lysates. Western blot analyses showed that all the recombinant proteins were immunologically reactive to rabbit polyclonal antibodies and to a mouse monoclonal antibody...
Topics
- Allergens
- Blotting, Western
- DNA, Complementary
- DNA, Plant
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Mutation
- Oryza
- Seeds
- Terminology as Topic
- Trypsin Inhibitors