Article
Purification, identification and preliminary crystallographic characterization of a novel seed protein from Vigna unguiculata.
Acta crystallographica. Section D, Biological crystallography - 1 Nov 2004
Chanana Veenu, Kaur Kanwal J, Salunke Dinakar M
Abstract excerpt
A tropical legume, Vigna unguiculata, was explored in order to identify potential allergens among the abundant seed proteins and to attempt their crystallographic study. Salt fractionation of the seed extract followed by chromatographic separation led to the purification of a 25 kDa protein. Gel-filtration chromatography of the 80% ammonium sulfate precipitation fraction led to separation of this protein in pure...
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