Article
Targeted gene inactivation in petunia by PCR-based selection of transposon insertion mutants.
Proceedings of the National Academy of Sciences of the United States of America - 29 Aug 1995
Koes R, Souer E, van Houwelingen A, Mur L, Spelt C, Quattrocchio F, Wing J, Oppedijk B, Ahmed S, Maes T
Abstract excerpt
Establishment of loss-of-function phenotypes is often a key step in determining the biological function of a gene. We describe a procedure to obtain mutant petunia plants in which a specific gene with known sequence is inactivated by the transposable element dTph1. Leaves are collected from batches of 1000 plants with highly active dTph1 elements, pooled according to a three-dimensional matrix, and screened by...
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