Article
Petunia plants escape from negative selection against a transgene by silencing the foreign DNA via methylation.
Molecular & general genetics : MGG - 1 May 1992
Renckens S, De Greve H, Van Montagu M, Hernalsteens J P
Abstract excerpt
Transgenic Petunia hybrida clones harbouring the T-DNA gene 2 of Agrobacterium tumefaciens were used to test a strategy for the trapping of plant transposable elements. In the Petunia line used, floral variegation is due to the presence of the non-autonomous transposable element dTph1 at the An1...
Topics
- Agrobacterium tumefaciens
- Amidohydrolases
- Azacitidine
- Blotting, Southern
- Cell Line
- Chromosome Deletion
- Cloning, Molecular
- DNA Transposable Elements
- DNA, Bacterial
- Gene Expression Regulation, Enzymologic
- Methylation
- Nucleic Acid Hybridization
- Phenotype
- Plants, Genetically Modified
- Restriction Mapping
- Selection, Genetic
- Transformation, Genetic