Article
A general method to isolate genes tagged by a high copy number transposable element.
The Plant journal : for cell and molecular biology - 1 Apr 1995
Souer E, Quattrocchio F, de Vetten N, Mol J, Koes R
Abstract excerpt
The Petunia hybrida line W138 contains more than 200 copies of the transposable element dTph1. In W138 progeny these elements give rise to new unstable mutations at high frequency. With the aim of isolating these mutated genes a method was developed to isolate dTph1 flanking sequences unique for...
Topics
- Alleles
- Base Sequence
- Cloning, Molecular
- DNA Primers
- DNA Transposable Elements
- DNA, Plant
- Gene Amplification
- Genes, Plant
- Genetic Techniques
- Genetic Vectors
- Molecular Sequence Data
- Mutation
- Plants
- Polymerase Chain Reaction
- Sequence Tagged Sites