Article
cDNA analysis of the mite allergen Lep d 1 identifies two different isoallergens and variants.
FEBS letters - 14 Aug 1995
Schmidt M, Olsson S, van der Ploeg I, van Hage-Hamsten M
Abstract excerpt
For the first time the complete cDNA encoding two isoallergens of the non-pyroglyphid dust mite Lepidoglyphus destructor, Lep d 1, allergen has been sequenced. In addition, one of the isoallergens was found to have two variants. Oligonucleotides were designed from known amino acid sequences. The cDNA sequences were obtained by hybridizing these primers to mRNA and enhancement by the RT-PCR technique. To obtain...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- DNA Primers
- DNA, Complementary
- Genetic Variation
- Mites
- Molecular Sequence Data
- Polymerase Chain Reaction
- Protein Biosynthesis
- Proteins
