Article
Production of a chimeric fibroin light-chain polypeptide in a fibroin secretion-deficient naked pupa mutant of the silkworm Bombyx mori.
Journal of molecular biology - 11 Aug 1995
Mori K, Tanaka K, Kikuchi Y, Waga M, Waga S, Mizuno S
Abstract excerpt
The allelic Nd-s and Nd-sD mutations of the silkworm Bombyx mori are mapped to the same locus as that of the fibroin light (L)-chain gene (Fib-L). The silkworm carrying the homozygous Nd-s or Nd-sD mutation secretes less than 0.3% of the normal level of fibroin and produces a thin cocoon (naked pupa). In this study, cDNA sequences of the Nd-s and Nd-sD L-chains were compared with the cDNA and genomic sequences of...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Southern
- Blotting, Western
- Bombyx
- Cloning, Molecular
- DNA, Complementary
- Disulfides
- Electrophoresis, Gel, Two-Dimensional
- Exons
