Article
Two mutations in the HMG-box with very different structural consequences provide insights into the nature of binding to four-way junction DNA.
The EMBO journal - 1 Aug 1995
Teo S H, Grasser K D, Hardman C H, Broadhurst R W, Laue E D, Thomas J O
Abstract excerpt
Mutation of the highly conserved tryptophan residue in the A-domain HMG-box of HMG1 largely, but not completely, destroys the protein tertiary structure and abolishes its supercoiling ability, but does not abolish structure-specific DNA binding to four-way junctions. Circular dichroism shows that the protein has some residual alpha-helix (< 10%) and does not re-fold in the presence of DNA. Structure-specific DNA...
Topics
- Amino Acid Sequence
- Base Sequence
- Circular Dichroism
- DNA
- DNA, Superhelical
- High Mobility Group Proteins
- Magnetic Resonance Spectroscopy
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation