Article
Two mutations in the HMG-box with very different structural consequences provide insights into the nature of binding to four-way junction DNA.
The EMBO journal - 1 Aug 1995
Teo S H, Grasser K D, Hardman C H, Broadhurst R W, Laue E D, Thomas J O
Abstract excerpt
Mutation of the highly conserved tryptophan residue in the A-domain HMG-box of HMG1 largely, but not completely, destroys the protein tertiary structure and abolishes its supercoiling ability, but does not abolish structure-specific DNA binding to four-way junctions. Circular dichroism shows that the protein has some residual alpha-helix (< 10%) and does not re-fold in the presence of DNA. Structure-specific DNA...
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