Applications of the polymerase chain reaction (PCR) in diagnosis.

Abstract excerpt

This paper reports on three applications of the polymerase chain reaction (PCR) in the pathology laboratory, cystic fibrosis carrier status investigation, Staphylococcus aureus identification and HLA-DQ alpha tissue typing. Allele specific PCR was used to detect the common cystic fibrosis mutation (delta F508) in an Irish family. The genomic status of each member was shown to be either heterozygous (carrier) or...

Topics

• Alleles
• Base Sequence
• Cystic Fibrosis
• DNA
• Female
• HLA-DQ Antigens
• Heterozygote
• Humans
• Male
• Molecular Sequence Data
• Mutation
• Polymerase Chain Reaction