Article
Molecular cloning and expression of a mouse thiamin pyrophosphokinase cDNA.
The Journal of biological chemistry - 26 Nov 1999
Nosaka K, Onozuka M, Nishino H, Nishimura H, Kawasaki Y, Ueyama H
Abstract excerpt
Thiamin pyrophosphokinase (EC 2.7.6.2) catalyzes the pyrophosphorylation of thiamin with adenosine 5'-triphosphate to form thiamin pyrophosphate. A mouse thiamin pyrophosphokinase cDNA clone (mTPK1) was isolated using a combination of mouse expressed sequence tag database analysis, a two-step polymerase chain reaction procedure, and functional complementation screening with a Saccharomyces cerevisiae thiamin...
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