Article
Liver fructose-1,6-bisphosphatase cDNA: trans-complementation of fission yeast and characterization of two human transcripts.
Differentiation; research in biological diversity - 1 Jul 1995
Bertolotti R, Armbruster-Hilbert L, Okayama H
Abstract excerpt
The SV40 early promoter is active both in mammalian cells and in the fission yeast Schizosaccharomyces pombe, and is used to drive full-length cDNA in polyvalent pcD-libraries. Two such liver libraries, of human and rat origin, were used to trans-complement a S. pombe mutant deficient in fructose...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- DNA, Complementary
- Down-Regulation
- Fructose-Bisphosphatase
- Genetic Complementation Test
- Gluconeogenesis
- Humans
- Insulin
- Intestinal Mucosa
- Intestines
- Kidney
- Liver
- Middle Aged
- Molecular Sequence Data
- Mutation