Isolation and characterization of an allelic cDNA for human muscle fructose-1,6-bisphosphatase.

doi:10.1016/s0378-1119(98)00181-4

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Isolation and characterization of an allelic cDNA for human muscle fructose-1,6-bisphosphatase.

Gene - 8 Jun 1998

Tillmann H, Eschrich K

Abstract excerpt

By applying a newly developed method, cDNAs for the human muscle isoform of fructose-1,6-bisphosphatase were isolated from phage- and plasmid-derived libraries. From these cDNAs and an EST clone, a composite sequence (1302 bp) was deduced that contains an open reading frame encoding a polypeptide of 339 amino acids with an estimated molecular weight of 36 755. After overexpression in E. coli, recombinant human...

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Isolation and characterization of an allelic cDNA for human muscle fructose-1,6-bisphosphatase.

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Isolation and characterization of an allelic cDNA for human muscle fructose-1,6-bisphosphatase.

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