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Article

Transfection and continuous expression of heterologous genes in the protozoan parasite Entamoeba histolytica.

12 Sept 1995

Abstract excerpt

To provide tools for functional molecular genetics of the protozoan parasite Entamoeba histolytica, we investigated the use of the prokaryotic neomycin phosphotransferase (NEO) gene as a selectable marker for the transfection of the parasite. An Escherichia coli-derived plasmid vector was constructed (pA5'A3'NEO) containing the NEO coding region flanked by untranslated 5' and 3' sequences of an Ent. histolytica...

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PMID: 7568055
DOI: 10.1073/pnas.92.19.8975

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