Insulin-Like Growth Factor-I and -II Binding and Action on DNA Synthesis in Rainbow Trout Spermatogonia and Spermatocytes1

Abstract excerpt

Radio-labeled recombinant human insulin-like growth factor (125I-rhIGF-I) bound specifically to total testicular cells and to spermatogonia plus primary spermatocytes (Go+CI) that had been prepared from trout testes at various maturation stages by centrifugal elutriation and then cleared of somatic cells by preculture in the presence of 2% Ultroser G. Binding sites showed high affinity (Ka = 0.5 +/- 0.2 x 10(10)...

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