Article
Characteristics of trophoblast cells migrating from first trimester chorionic villus explants and propagated in culture.
Placenta - 1 Jul 1995
Irving J A, Lysiak J J, Graham C H, Hearn S, Han V K, Lala P K
Abstract excerpt
We developed a method of propagating pure first trimester human trophoblast cells growing out of primary explants of mechanically derived chorionic villus fragments (Yagel et al, 1989; Graham et al, 1992). We have now extensively characterized these cells during their initial outgrowth and in long-term culture, employing a variety of markers and techniques as outlined below. By double label immunofluorescence...
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