Article
[Isolation of intact supercoiled mitochondrial DNA from the yeast Saccharomyces cerevisiae].
Molekuliarnaia biologiia - 1 Jan 2000
Larionov V L, Aver'ianov A V, Gauze G G
Abstract excerpt
Cells of Saccharomyces cerevisiae strain PG-60 were mechanically disrupted in the presence of ethidium bromide, and the total covalently closed circular DNA (ccDNA) was prepared by EthBr-CsCl equilibrium centrifugation of lysed 15 g pellet of the homogenate. Recentrifugation of ccDNA in equilibrium neutral CsCl density gradient resolved a discrete light peak (LccDNA) with the buoyant density equal to 1.684 g/cm3...
Topics
- Centrifugation, Density Gradient
- DNA, Circular
- DNA, Mitochondrial
- DNA, Superhelical
- Microscopy, Electron
- Mutation
- Nucleic Acid Hybridization
- Saccharomyces cerevisiae
- Transcription, Genetic