Label-free Raman spectroscopic imaging enables high-content molecular phenotyping of respiratory diseases from bronchoalveolar lavage fluid.

Abstract excerpt

BACKGROUND: Conventional BALF analysis is limited by time-consuming labeling, poor multiplexing, and lack of spatial resolution-constraints that Raman imaging can overcome through label-free, multiplexed molecular mapping. However, its clinical application to BALF remains unexplored. We aimed to address this gap by establishing a Raman imaging platform for simultaneous biomarker detection across respiratory...

Topics

• Spectrum Analysis, Raman
• Humans
• Bronchoalveolar Lavage Fluid
• Phenotype
• Biomarkers
• Respiratory Tract Diseases