Article
Establishment of a CRISPR-Cas9 Library for Indica Rice and Identification of OsOPR5 (LOC_Os06g11210) as a Regulator of Root Architecture.
Physiologia plantarum - 1 Jan 2026
Chowdhury Sankhadeep, Nayak Suprava Priyadarsini, Pattanayak Rajashree, Sardar Shaswati, Majhi Ashis, Yadav Banita, Dasari Abhilash, Mandlik Rushil, Sonah Humira, Deshmukh Rupesh, Gupta Ishaan, Bauer Petra, Ram Hasthi
Abstract excerpt
Functional characterization of a large number of rice genes remains a major challenge despite the availability of genome sequences and large-scale transcriptomic datasets. CRISPR-Cas9 library is a powerful approach for high-throughput targeted mutagenesis; however, its application in indica rice cultivars remains limited due to low transformation and regeneration efficiencies. In this study, we developed a...
Read the complete abstract on PubMedTopics
- Oryza
- CRISPR-Cas Systems
- Plant Roots
- Plant Proteins
- Plants, Genetically Modified