Article
Noninvasive Detection of Bladder Cancer via Urinary DNA Mutation and Methylation Profiling Using Modified Real-Time Quantitative PCR.
The Journal of molecular diagnostics : JMD - 1 Oct 2026
Jin Di, Wang Hailong, Jiang Chen, Zhang Fengwu, Wang Xinchang, Zhu Miaojun, Wu Hongfei, Cong Qing, Han Jie, Zhu Zirui, Xue Jingdong, Qian Lei, Cao Ming, Zhang Lianhua, Xue Wei, Huang Yiran, Tan Ruoying, Zhang Ruiyun, Chen Haige
Abstract excerpt
Cystoscopy is the gold standard for bladder cancer (BC) diagnosis and surveillance but experiences invasiveness and patient discomfort. Existing urine-based assays with single-gene mutation or methylation biomarkers exhibit insufficient diagnostic efficacy, necessitating a reliable noninvasive detection approach. This study developed EpiMut-BC, a high-specificity urinary PCR assay integrating DNA mutation and...
Read the complete abstract on PubMedTopics
- Humans
- Female
- Urinary Bladder Neoplasms
- DNA Methylation
- Mutation