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High-resolution melting (HRM)-based genotyping of Toxoplasma gondii in meat products: comparative evaluation of ROP18, ROP5, and B1 gene markers.

World journal of microbiology & biotechnology - 29 Jun 2026

Fotouhi-Ardakani Reza, Sebt-Ahmadi Khadije, Mosawi Sayed-Hussain, Zarean Mehdi, Afgar Ali, Fotouhi-Ardakani Rezvan, Moradzadeh Zahra, Afkhami Hamed

Abstract excerpt

Consumption of raw or undercooked meat is a major route of transmission of Toxoplasma gondii (T. gondii) to humans worldwide. This study aimed to develop and evaluate high-resolution melting (HRM) assays targeting two novel polymorphic regions of the rhoptry protein 18 (ROP18) gene (106-bp and 125-bp) for genotyping T. gondii in meat products, in comparison with the B1 and ROP5 genes. A total of 64 samples,...

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PMID: 42371201
DOI: 10.1007/s11274-026-05107-5

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