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[Construction and verification of infectious clones for human respiratory syncytial virus subgroup A genotype ON1 expressing fluorescent reporter genes].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] - 6 Jun 2026

Shi Y Q, Wang N, Mao N Y, Zhu Z, Hu H Q, Song J J, Xie Z B, Cao L, Song J H, Jiang J, Zhu W Y, Zhang Y

Abstract excerpt

Objective: To construct a reverse genetic system for the genotype ON1 of human respiratory syncytial virus subtype A (HRSV-A) expressing fluorescent reporter genes. Methods: Recombinant plasmids encoding EGFP or mCherry were constructed based on the 2019 Beijing HRSV-A ON1 dominant strain (6914). Recombinant viruses, rescued by co-transfecting BSR/T7-9 cells with helper plasmids, were identified via indirect...

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PMID: 42331539
DOI: 10.3760/cma.j.cn112150-20260305-00174

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