Article
Simultaneous detection of multiple sdhB SNPs in Botrytis cinerea using a universal quenching probe-based LAMP assay.
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry - 1 Sept 2026
Fukuta Shiro, Kojima-Ando Hiroko, Mori Ken-Ichiro, Hara Tomoaki, Tsuzuki Masami, Inagaki Reina, Tsunekawa Kenta, Uchida Yuta, Arakawa Mizuho, Yamada Kensuke
Abstract excerpt
Succinate dehydrogenase inhibitors (SDHIs) are widely used to control Botrytis cinerea, but resistance caused by multiple point mutations in the sdh genes has become a major concern. To enable rapid and accurate identification of SDHI‑resistant genotypes, we developed a loop‑mediated isothermal amplification (LAMP) assay using a universal quenching probe (UQProbe LAMP) capable of simultaneously detecting multiple...
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