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Loop-Mediated Isothermal Amplification (LAMP) Genotyping for Presence-Absence Determination of Gene of Interest in Test Samples.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2025

Torres Anthony, Gaudino Reginald

Abstract excerpt

This chapter explores the use of loop-mediated isothermal amplification (LAMP) for rapid and cost-effective genotyping, highlighting its advantages in field-based or resource-limited settings due to its isothermal nature. DNA is extracted from FTA samples and leaf tissue for input into a downstream LAMP assay targeting specific genes. The method's high sensitivity and specificity enables accurate detection of...

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PMID: 40580285
DOI: 10.1007/978-1-0716-4642-7_5

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