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An Illumina-based amplicon sequencing approach designed to determine grapevine fanleaf virus isolates.

Scientific reports - 13 Jun 2026

Martin Isabelle R, Mustin Pierre, Rhalloussi Wassim, Garcia Shahinez, Hagege Myriam, Kubina Julie, Vigne Emmanuelle, Hily Jean-Michel

Abstract excerpt

Traditionally, to comprehensively assess the genetic diversity of a viral isolate, i.e. the population of variants, many molecular steps are necessary, mostly involving PCR amplification, cloning and Sanger sequencing. Lately, with its advent, high throughput sequencing (HTS) has become the method of choice. Both approaches, while extremely informative, may not be economically advantageous to use on a large...

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PMID: 42288550
DOI: 10.1038/s41598-026-53623-z
PMCID: PMC13522481

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