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Detection and genetic variation analysis of grapevine fanleaf virus (GFLV) isolates in China.

Archives of virology - 1 Nov 2015

Zhou Jun, Fan Xudong, Dong Yafeng, Zhang Zun Ping, Ren Fang, Hu Guojun

Abstract excerpt

To investigate the prevalence and genetic variation of grapevine fanleaf virus (GFLV) in China, 142 grapevine samples from 13 provinces and regions were tested using DAS-ELISA, RT-PCR, and nested RT-PCR. Of the samples, 38% tested positive for GFLV by DAS-ELISA, and 26.8% tested positive by RT-PCR and nested RT-PCR. Movement protein (MP) and coat protein (CP) gene PCR products were cloned and sequenced. The MP or...

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PMID: 26264404
DOI: 10.1007/s00705-015-2566-0

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