How wastewater composition and isolation workflow shape Raman-based bacterial phenotyping.

Abstract excerpt

Wastewater represents a structurally and chemically heterogeneous environment in which suspended particulates and polymeric organic matter influence microbial recovery and measurable phenotype. Here, we systematically examined how matrix architecture and sample handling affect single-cell Raman phenotyping. Using defined synthetic wastewater supplemented with either agarose (structurally stable) or tapioca...

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