Article
Label-free Raman characterization of bacteria calls for standardized procedures.
Journal of microbiological methods - 1 Aug 2018
García-Timermans Cristina, Rubbens Peter, Kerckhof Frederiek-Maarten, Buysschaert Benjamin, Khalenkow Dmitry, Waegeman Willem, Skirtach Andre G, Boon Nico
Abstract excerpt
Raman spectroscopy has gained relevance in single-cell microbiology for its ability to detect bacterial (sub)populations in a non-destructive and label-free way. However, the Raman spectrum of a bacterium can be heavily affected by abiotic factors, which may influence the interpretation of experimental results. Additionally, there is no publicly available standard for the annotation of metadata describing sample...
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