Enterovirus Testing in Hand, Foot, and Mouth Disease and Herpangina: A Highly Sensitive Single-Round VP4-VP2 Reverse-Transcription Polymerase Chain Reaction Assay with a Redesigned Reverse Primer.

Abstract excerpt

Molecular typing of enteroviruses (EVs) is essential for surveillance of hand, foot, and mouth disease (HFMD). Conventional reverse-transcription polymerase chain reaction (RT-PCR) targeting the VP4-VP2 region can be insufficiently sensitive, reducing the detectability of Enterovirus A (EV-A). We developed a single-round RT-PCR assay using a modified reverse primer design (C3R) for rapid EV detection and...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Computer Simulation
• Share to DNA Primers