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Article

Innovative CRISPR/Cas9-Based Strategy for Allele-Specific HLA Peptidome Analysis Using a Pan-HLA Antibody.

Molecular & cellular proteomics : MCP - 1 Jun 2026

Cobos-Figueroa Laura, Pintor-Poveda Ana, Mir Carmen, Melamed Dganit, Admon Arie, Lauzurica Pilar, Lorente Elena

Abstract excerpt

Human leukocyte antigen (HLA) immunopeptidomics is restricted by the limited availability of allele-specific antibodies and by potential artifacts introduced by HLA overexpression systems. To address these challenges, we developed a CRISPR/Cas9-based strategy that selectively deletes undesired classical class I alleles while preserving a single endogenous allele, thereby enabling allele-resolved peptidome...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 42069060
DOI: 10.1016/j.mcpro.2026.101578
PMCID: PMC13240811

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

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