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Article

X-ray and biochemical analysis of N370S mutant human acid β-glucosidase.

The Journal of biological chemistry - 7 Jan 2011

Wei Ronnie R, Hughes Heather, Boucher Susan, Bird Julie J, Guziewicz Nicholas, Van Patten Scott M, Qiu Huawei, Pan Clark Qun, Edmunds Tim

Abstract excerpt

Gaucher disease is caused by mutations in the enzyme acid β-glucosidase (GCase), the most common of which is the substitution of serine for asparagine at residue 370 (N370S). To characterize the nature of this mutation, we expressed human N370S GCase in insect cells and compared the x-ray structure and biochemical properties of the purified protein with that of the recombinant human GCase (imiglucerase,...

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Identifiers

PMID: 20980263
DOI: 10.1074/jbc.M110.150433
PMCID: PMC3012987

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