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CRISPR/Cas9 engineered and whole-genome characterized KIT D816V-mutant human iPSC lines.

Stem cell research - 1 Jun 2026

Luo Yanyan, Vallone Valeria Fernandez, Blanc Eric, Miller Duncan C, He Jiajun, Stachelscheid Harald, Beule Dieter, Scheffel Jörg, Siebenhaar Frank

Abstract excerpt

We report on the generation of human induced pluripotent stem cell (iPSC) lines, BIHi005-A-86 and BIHi005-A-87, carrying the KIT D816V mutation associated with Indolent Systemic Mastocytosis (ISM). To overcome the confounding genetic backgrounds of existing leukemic models, we introduced this gain-of-function mutation into the healthy BIHi005-A line using CRISPR/Cas9 editing. The resulting clones were validated...

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Identifiers

PMID: 41916193
DOI: 10.1016/j.scr.2026.103975

Genes

KIT

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

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