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Directed evolution of a serine protease LsPLAase in Bacillus subtilis for high-yield secretory production and efficient depolymerization of polylactic acid.

Bioresource technology - 1 Jun 2026

Cheng Xiao-Tao, Wu Lei, Wang Wenyao, Wu Ting, Xie Bin, Yan Xin, Dong Weiliang, Zhou Jie, Jiang Min

Abstract excerpt

The bio-recyclability of polylactic acid (PLA) is hampered by deficient capacity and low yields of the depolymerases. Whether native production in original isolate Laceyella sacchari LP175 or preparation using Escherichia coli system, the poly(l-lactide) (PLLA) depolymerase, LsPLAase was restricted for industrial applications by complex downstream purification and limited protein yields. Herein, based on...

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PMID: 41861863
DOI: 10.1016/j.biortech.2026.134441

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