Lumi-Map, a Luciferase Bioluminescence Screen of Mutants Combined with MutMap Allows Rapid Dissection of Plant Immune Signaling Pathways.

Abstract excerpt

Genetic screening of mutants and identification of their causative genes are effective strategies for understanding plant signaling pathways. Here, we present Lumi-Map, a method that combines real-time luciferase bioluminescence monitoring with MutMap analysis to identify single nucleotide polymorphisms (SNPs) responsible for the mutant phenotypes. Real-time monitoring of reporter gene expression enables...

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