Article
Genome sequencing reveals agronomically important loci in rice using MutMap.
Nature biotechnology - 22 Jan 2012
Abe Akira, Kosugi Shunichi, Yoshida Kentaro, Natsume Satoshi, Takagi Hiroki, Kanzaki Hiroyuki, Matsumura Hideo, Yoshida Kakoto, Mitsuoka Chikako, Tamiru Muluneh, Innan Hideki, Cano Liliana, Kamoun Sophien, Terauchi Ryohei
Abstract excerpt
The majority of agronomic traits are controlled by multiple genes that cause minor phenotypic effects, making the identification of these genes difficult. Here we introduce MutMap, a method based on whole-genome resequencing of pooled DNA from a segregating population of plants that show a useful phenotype. In MutMap, a mutant is crossed directly to the original wild-type line and then selfed, allowing...
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