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Article

Accurate variant effect estimation in FACS-based deep mutational scanning data with Lilace.

Genome biology - 27 Jan 2026

Freudenberg Jerome, Rao Jingyou, Howard Matthew K, Macdonald Christian, Greenwald Noah F, Coyote-Maestas Willow, Pimentel Harold

Abstract excerpt

Deep mutational scanning (DMS) coupled with fluorescence-activated cell sorting (FACS) provides a high-throughput method to link genetic variants with quantitative molecular phenotypes. Analysis of these experiments is challenging due to measurement variance and the multidimensional FACS readout. However, no statistical method has yet been developed to address these challenges. Here we present Lilace, a Bayesian...

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PMID: 41593735
DOI: 10.1186/s13059-026-03934-1
PMCID: PMC12918140

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