Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Optimized Method for Establishing Primary Human Mesothelial Cell Cultures Preserving Epithelial Phenotype.

Biomolecules - 30 Nov 2025

Kuznetsova Evdokiya, Bakalenko Nadezhda, Gaifullina Liana, Atyukov Mikhail, Dergilev Konstantin, Beloglazova Irina, Malashicheva Anna

Abstract excerpt

Mesothelial cells (MCs) are highly relevant for studying the pathogenesis of serosal diseases, fibrosis, inflammation, and tumor progression. However, the isolation and maintenance of an epithelial-like phenotype of MCs in vitro remain methodologically challenging due to their tendency to undergo mesothelial-to-mesenchymal transition (MMT). In this work, we propose a combined protocol utilizing collagen IV...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cell Culture Techniques
Share to Cell Survival

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Cells, Cultured

Share to Culture Media, Conditioned

Share to Epithelial Cells

Share to Epithelial-Mesenchymal Transition

Share to Primary Cell Culture

Share to rho-Associated Kinases

Source

Open in PubMed Open full text

Identifiers

PMID: 41463325
DOI: 10.3390/biom15121669
PMCID: PMC12730630

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Optimized Method for Establishing Primary Human Mesothelial Cell Cultures Preserving Epithelial Phenotype.PMID 41463325Read ↗

Peritoneal Submesothelial Stromal Cells Support Hematopoiesis and Differentiate into Osteogenic and Adipogenic Cell Lineages.Semantic proximity Read ↗

Characterisation of Cultured Mesothelial Cells Derived from the Murine Adult OmentumSemantic proximity Read ↗

An Optimized Method to Isolate Human Fibroblasts from Tissue for Ex Vivo AnalysisSemantic proximity Read ↗

Pleura Organoid Transplantation Facilitates Visceral Pleural Repair through CD133+ Progenitor Cell-Mediated RegenerationSemantic proximity Read ↗

Isolation of Primary Myofibroblasts from Mouse and Human Colon TissueSemantic proximity Read ↗

Use of a Collagen Membrane to Enhance the Survival of Primary Intestinal Epithelial Cells.Semantic proximity Read ↗

Isolation of CD 90+ Fibroblast/Myofibroblasts from Human Frozen Gastrointestinal Specimens.Semantic proximity Read ↗

A protocol for the culture and isolation of murine synovial fibroblastsSemantic proximity Read ↗

Identification and prospective isolation of a mesothelial precursor lineage giving rise to smooth muscle cells and fibroblasts for mammalian internal organs, and their vasculature.Semantic proximity Read ↗

Phenotypic and functional characteristics of porcine peritoneal mesothelial cells.Semantic proximity Read ↗

A Promising transwell co-culture cell model for silicosisSemantic proximity Read ↗

Isolation and Primary Culture of Various Cell Types from Whole Human Endometrial BiopsiesSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.