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Article

Reverse BSA-QTLseq: A new genotype-driven bioinformatics approach for simultaneous trait mapping.

Plant communications - 12 Jan 2026

Esposito Salvatore, D'Agostino Nunzio, Taranto Francesca, Fania Fabio, Pavan Stefano, Colella Ida, Sestili Francesco, Lafiandra Domenico, De Vita Pasquale

Abstract excerpt

Bulked segregant analysis (BSA) is a widely used method for identifying genomic loci associated with traits of interest in crops. However, conventional BSA is limited by its reliance on phenotype-driven bulk sampling, which restricts its scalability and confines its applicability to single-trait analysis. This study introduces a novel method, reverse BSA-QTLseq, which uses genotype-driven bulk reconstruction...

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Identifiers

PMID: 41206515
DOI: 10.1016/j.xplc.2025.101588
PMCID: PMC12902257

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