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Evaluation of an MPS-Based Microhaplotype Assay for Non-Invasive Prenatal Paternity Testing.

Electrophoresis - 1 Oct 2025

Zhang Ranran, Xue Jiaming, Tan Mengyu, Yang Fan, Liu Guihong, Zheng Yazi, Wu Qiushuo, Liao Miao, Lv Meili, Li Chengtao, Zhang Suhua, Qu Shengqiu, Liang Weibo

Abstract excerpt

Non-invasive prenatal paternity testing (NIPPT) enables the genotyping of cell-free DNA (cfDNA) from maternal plasma through deep sequencing. Microhaplotypes (MHs) combine the advantages of short tandem repeats (STRs) and single-nucleotide polymorphisms (SNPs) and have attracted much attention in NIPPT. In this study, we optimized 45 MHs from our previous study and confirmed the effectiveness of the 45plex MH...

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PMID: 40959999
DOI: 10.1002/elps.70021

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