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Article

Engineered Membrane Vesicle Production via oprF or oprI Deletion Has Distinct Phenotypic Effects in Pseudomonas putida.

ACS synthetic biology - 18 Jul 2025

Wilkes Rebecca A, Miller Tarryn E, Waldbauer Jacob, Zhou Nanqing, Zhang Lichun, DiBiase Beth N, Kamat Neha P, Aristilde Ludmilla, Beckham Gregg T, Werner Allison Z

Abstract excerpt

Membrane vesicle (MV) production is a natural phenomenon in Gram-negative bacteria and represents an emerging synthetic biology tool for the secretion of biomolecules or bioproducts. Manipulation of membrane components has proven successful in enhancing MV production. However, the impact of membrane disruptions on strain fitness and protein composition warrants further investigation for the use of MVs in...

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PMID: 40607986
DOI: 10.1021/acssynbio.5c00171
PMCID: PMC12281618

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