Article
[Non-enzymatic DNA methylation by S-adenosylmethionine results in the formation of minor thymine residues and 5-methylcytosine from cytosine].
Molekuliarnaia biologiia - 1 Jan 2000
Mazin A L, Gimadutdinov O A, Turkin S I, Burtseva N N, Vaniushin B F
Abstract excerpt
It was found that nonenzymatic DNA methylation proceeds in water solution in the presence of S-adenosylmethionine (AdoMet). The main reaction products are thymine and 5-methylcytosine residues. It was shown that labelled thymine residues are formed also upon DNA incubation in the presence of [methyl-14C]methionine as well as [methyl-14C]cobalamine. Only cytosine reacts with AdoMet resulting in thymine production....
Topics
- 5-Methylcytosine
- Animals
- Base Sequence
- Cattle
- Chromatography, High Pressure Liquid
- Chromatography, Thin Layer
- Cytosine
- DNA
- In Vitro Techniques
- Methionine
- Methylation