Article
The bacterial transcription terminator, Rho, functions as an RNA:DNA hybrid (RDH) helicase in vivo.
The Biochemical journal - 26 May 2025
Bhosale Ankita, Sen Ranjan
Abstract excerpt
Ribonuclease HI (rnhA) removes the deleterious RNA:DNA hybrids (RDHs) by cleaving its RNA component. The bacterial transcription terminator Rho is an RNA-dependent 5' → 3' helicase capable of unwinding RDH formed on a single-stranded RNA in vitro. We hypothesize that Rho might be directly involved in RDH removal in vivo. Here, we demonstrate that Rho primary RNA-binding site (PBS) mutants defective in RNA binding...
Topics
- Escherichia coli
- Ribonuclease H
- Escherichia coli Proteins
- Rho Factor
- RNA, Bacterial
- DNA Helicases
- DNA, Bacterial
- Mutation