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Comparison of sample pretreatments used to distinguish between infectious and non-infectious foodborne viruses by RT-qPCR

22 Feb 2025

Abstract excerpt

), magnetic silica beads and centrifugal filter using HAV or HuNoV inactivated by heat, pulsed light, or sodium hypochlorite (NaOCl). PMAxx completely or nearly eliminated (3.96 ± 1.24 log gc) the RT-qPCR signal of HAV inactivated at 100°C for 10 min. Pretreatments could not reduce significantly RT-qPCR signal of HAV after pulsed light (0.74 ± 0.36 log gc) and NaOCl (0.24 ± 0.14 log gc) inactivation. Enzymatic...

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PMID: 39993658
DOI: 10.1016/j.jviromet.2025.115130

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