Performance of pre-RT-qPCR treatments to discriminate infectious human rotaviruses and noroviruses from heat-inactivated viruses: applications of PMA/PMAxx, benzonase and RNase

Abstract excerpt

Detection/Quantification of RNA viruses is mostly done by reverse‐transcriptase (RT)‐(q)PCR, but it does not distinguish between infectious and noninfectious viruses. Our aim was to test, how different pretreatments before RT‐qPCR could eliminate positivity originated from external nucleic acids or genomes of damaged particles. Heat‐inactivated (80°C for 10 min) rotavirus Wa strain and faecal samples containing...

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