Article
Combinatorial genetic transformation generates a library of metabolic phenotypes for the carotenoid pathway in maize.
Proceedings of the National Academy of Sciences of the United States of America - 25 Nov 2008
Zhu Changfu, Naqvi Shaista, Breitenbach Jürgen, Sandmann Gerhard, Christou Paul, Capell Teresa
Abstract excerpt
Combinatorial nuclear transformation is a novel method for the rapid production of multiplex-transgenic plants, which we have used to dissect and modify a complex metabolic pathway. To demonstrate the principle, we transferred 5 carotenogenic genes controlled by different endosperm-specific promoters into a white maize variety deficient for endosperm carotenoid synthesis. We recovered a diverse population of...
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