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Multiplex allele-specific PCR and DNA chromatography to identify genotypes of Salmonella enterica serovar Typhimurium and its monophasic variants.

Journal of microbiological methods - 1 Jan 2025

Arai Nobuo, Tamamura-Andoh Yukino, Iwata Taketoshi, Watanabe-Yanai Ayako, Kusumoto Masahiro

Abstract excerpt

We constructed a simple genotyping method combining multiplex allele-specific PCR and DNA chromatography for Salmonella enterica subsp. enterica serovar Typhimurium and its monophasic variant. The developed method can be used to estimate the genetic background of isolates, and it facilitates easy identification of several epidemic clades among these serotypes.

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Alleles
Chromatography
DNA, Bacterial
Genotyping Techniques
Multiplex Polymerase Chain Reaction

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Salmonella Infections

Salmonella typhimurium

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PMID: 39710120
DOI: 10.1016/j.mimet.2024.107082

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