Article
Comparison of multilocus variable-number tandem repeat analysis and pulsed-field gel electrophoresis in molecular subtyping of Salmonella enterica serovars Paratyphi A.
Diagnostic microbiology and infectious disease - 1 Jan 2011
Tien Yung-Yen, Wang You-Wun, Tung Sheng Kai, Liang Shiu-Yun, Chiou Chien-Shun
Abstract excerpt
Salmonella enterica serotype Paratyphi A is a highly clonal organism; pulsed-field gel electrophoresis (PFGE) is insufficient in discriminating isolates. A multilocus variable-number tandem repeat analysis (MLVA) was developed, and its usefulness in discriminating isolates was compared. PFGE analysis with XbaI and BlnI discriminated 55 isolates into 14 types, with a discriminatory index (DI) of 0.741 (confidence...
Topics
- Asia, Southeastern
- DNA, Bacterial
- Deoxyribonucleases, Type II Site-Specific
- Electrophoresis, Gel, Pulsed-Field
- Genotype
- Humans
- Minisatellite Repeats
- Molecular Typing
- Salmonella paratyphi A
- Sensitivity and Specificity